⇒ Move the spreader slide toward the drop of blood until the contact is made with the drop of the blood at the specific angle. There are many reasons why your doctor may order a blood smear. Preparing the Peripheral Blood Film . This article highlights the basic science and art behind the PBF. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. If you continue browsing the site, you agree to the use of cookies on this website. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. The drop should be in the center line of the glass slide. BLOOD COLLECTION BY VENIPUNCTURE – SYRINGE AND VACUTAINER METHOD. How to Make a Peripheral Blood Smear. The most common technique of blood smear preparation is called the “wedge or push” technique. The blood smear in Figure 3C results when a slight amount of the blood droplet is … Check out How to collect the blood sample by Venipuncture? Finger Prick or . 3. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. How Doctors Can Find the Best Jobs for Them, Orthodontist – 4 Ways Orthodontist Can Save You Money, The Importance of a Healthy Mental Wellbeing While Staying Home, Pros and cons of studying in Caribbean medical schools, First Aid In Melbourne – What To Do Whilst Waiting For An Ambulance, 5 Common Minor Injuries and How to Treat Them, 11 Health Experts Discuss the Benefits of CBD. Tnx for all keep it up am lab technician from Ethiopia. Blood smears are needed for microscopic examination of the blood. Maintain an even gentle pressure on the slide. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Place a small drop of blood, or one side about 1-2 cm from one end. Required fields are marked *. Capillary blood obtained by fingerstick: 1. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. associated with the decrease in the viscosity of blood. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. Part 1 - Preparation of Peripheral Blood Smear (PBS) - YouTube After staining the slide, the examination begins. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). Why Edibles Affect Differently Than Smoking Marijuana. Do not use a wax pencil or marker or any Pen as it dissolves and washed out during the staining process. MATERIALS REQUIRED FOR THICK PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THICK PERIPHERAL BLOOD SMEAR METHOD. of collection) Preparation of Blood Film: The slide should be clean. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. Preparation and staining of peripheral blood smear Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. Can Apple Cider Vinegar Benefit Your Hair? Precautions: Ensure that the whole drop of blood is picked up and spread. The page below is a sample from the LabCE course. (Babesiosis) etc. Preparation of blood smears . Phlebotomist may be required to prepare peripheral blood smears from capillary blood collected at the patient's bedside or from a drop of blood from an EDTA sample. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. The undiluted stain fixes and partially stains the smear. Peripheral Smear Preparation. CBC & Peripheral Blood Smear. Samples with heparin are unusable for preparation of the smears. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… Blood collection for thick or thin blood smears . Such an area for examination is illustrated in the image below. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. Blood films are examined in the investigation of hematological disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. A small drop of blood is placed on the midline at the end of a glass slide. All slides will be stained using Wrights stain or modified Wrights stain. ⇒ Allow the blood smear to air-dry completely. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. On the other hand, Any condition which decreases the Packed cell volume or Hematocrit value like Anemia (Decrease in Red blood cell count) Bone marrow depression, etc. This can most likely be observed at a site five or six oil fields from the end of the feathery portion (about 100 red cells per field). blood (within 1 hr. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. 2. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. Then move the spreader slide smoothly and rapidly forward over the specimen slide, drawing the blood behind it into a thin film that should be tongue-shaped. But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. Clean slides with 70 to 90% alcohol and allow to dry. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. A Thick blood film smear requires a large volume of blood as compared to Thin blood films Which enable the more efficient detection of parasites in the blood specimen. Thin smears. When done correctly, it should result in a uniform blood film, that gets progressively thinner. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. The site of examination is chosen; away from clumping, piling, or stacking of the red blood cells. In this video I quickly go over how to make a peripheral blood smear. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? ⇒ Using a lead pencil or glass marking pencil, write the Name, Identification no. Preparation of Blood Smear: Collection of Sample: 1. preparation of peripheral blood smear ⇒ Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. Your email address will not be published. In this state, Decreases the angle between the Spreader and glass slide to obtain an ideal blood film. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The blood smear is then fixed and stained with Romanowski stain for microscopic examination. Check out How to do a Capillary blood collection? This procedure is known as a blood film. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. At one time, a blood smear was prepared on nearly everyone who had a CBC. Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. Cover the blood smear with Leishman’s stain and keep it for 2 minutes. Preparation of a Peripheral Blood Film This protocol describes how to prepare a slide film of peripheral blood for microscopic examination. As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. Place the drop of blood in the center on one side of the glass slide leaving about 1 cm margins. Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. Cover the blood film with undiluted staining solution. The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. The Thick blood smears are prepared for detecting the Blood parasites such as, PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD, MATERIALS REQUIRED FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PRINCIPLE OF THICK PERIPHERAL BLOOD SMEAR METHOD. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. The angle of the spreader varies with the thickness or Viscosity of the blood to obtain an ideal smear. This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. The drop should be in the center line of the glass slide. Keep the same angle all the way to the end of the smear. Peripheral Blood Smear Preparation. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis The Thick blood smears are prepared for detecting the Blood parasites such as Plasmodium spp. Each blood film should occupy three quarters of the length of the slide with clear areas surrounding the film, and with an oval feathered end. White Blood Cell Differential Case Simulator, White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions, Learn more about White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (online CE course).  Smears should be made within 1 hour of blood collection from EDTA specimens stored at room temperature to avoid distortion of cell morphology  Blood smears can also be made from finger prick blood directly onto slide. Blood can also be taken from an EDTA sample. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a drop of blood from the tube onto the clean grease-free microscopic glass slide. Subscribe To Our Newsletter To Get All The Updates Right in Your Inbox, Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). Wear gloves. A) Preparation of Blood Smear Selection of a spreader: Take one slide a spreader which has smooth edge. Using a second slide as the spreader, the blood is streaked into a thin film in the tongue-like shape and allowed to dry. These margins are left onto the glass slide to get a region where the cells are spaced far enough apart to be counted and differentiated. E.D.T.A. Variations in Angle of Spreader for thin blood smear, PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY, Understanding the Costs of Cancer Treatment in Australia, Foods To Nourish Your Hair And Prevent Hair Loss, Priapism: The ED Medication Emergency You Should Know About, Essential First Aid Kit for the New Year Holiday Season, Patient Engagement: Ways to Stay Connected in Healthcare. I am a Medical Lab Tech, a Web Developer and Bibliophiliac. The blood smear is a simple blood test. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. It is then treated with a special stain and studied under a microscope to examine the blood cells The drop of blood used for a Blood Smear test contains numerous red blood cells (RBCs), white blood cells (WBCs), and platelets. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. Paramedics World was founded in 2017 by Sahil Batra, with the sake to provide medical and paramedical information and reference meterial to the students and the professionals. Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. Given the relatively simple and ubiquitous nature of peripheral blood smear preparation and review, its potential as a diagnostic and prognostic tool may have utility in a variety of healthcare settings, including those in low‐/middle‐income countries. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. Malaria Journal 2013; 12: 231. After staining the slide, the examination begins. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. Oral Hygiene: Good Practices for a Healthy Smile, Tips for Cleanroom Cleaning and Personal Protective Equipment (PPE), BEST AYURVEDIC HERBS TO FIGHT OFF STRESS & ANXIETY, Advantages of Using Cannabis Oil for Lung Cancer. Hi, I’m the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. Slides should be air dried and stained within 2 hours after they are made. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. Save my name, email, and website in this browser for the next time I comment. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. A thick blood smear is made by spreading a large blood drop in a small area of about 1 cm which provides a better opportunity to detect various parasitic forms against a more transparent background. (Malaria parasite),  Wuchereria bancrofti & Brugia malayi (Lymphatic filariasis), Leishmania donovani or other species of Leishmania (Leishmaniasis), Babesia spp. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. It expounds its la … The smear will be prepared either automatically by the LH Slidemaker, or manually, in the case of microtainers and samples that are not run in the primary mode on the Coulter and the ACP lab. The Blog is basically devoted to the Paramedical personnels who risk their life to save the life of other peoples. WEDGE BLOOD SMEAR • Specimen:  Peripheral blood smear made from EDTA- anticoagulated blood. 2. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). Normally, … ⇒ Collect the blood sample from the capillary or venipuncture. and the Date on the frosted end of the slide. For a blood smear, the lab professional looks for blood cell problems that may not be seen on a computer analysis. Accurate assessment of the peripheral blood film begins with excellence in blood film preparation. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a Large drop of blood from the tube onto the clean grease-free microscopic glass slide. Take one drop of venous or capillary blood. Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Start studying Preparation of Peripheral Blood Smear 3. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. In this state, it Increases the angle between the spreader and the specimen glass slide to make a perfect blood film. (adsbygoogle = window.adsbygoogle || []).push({}); Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Place the Blood drop in the center of the glass slide. At one time, a blood smear was prepared on nearly everyone who had a CBC. Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. Your email address will not be published. The conditions like Polycythemia (increase in the Red blood cell count), Dehydration or any condition which increases the Packed cell volume or Hematocrit value is associated with the increase in the viscosity of blood. Many blood tests use computers to analyze results. 6. Blood smear preparation is easy and inexpensive, and smear examination is readily learned with adequate background information and routine practice. 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The same angle all the way to the Paramedical personnels who risk their life to save the life other! Proper clarity s name ( or other identifier ), date and time of collection as soon as is. Abortion vs Medical Abortion: which one ’ s the Right Choice for you blood streaked! Stained as soon as it dissolves and washed out during the staining process staining protocol for staining blood according! In this browser for the next time I comment from its edges slide... With adequate background information and routine practice spreader slide on the slide midline at the end of a slide... When done correctly, it should result in a layer such that the thickness or Viscosity of the slide! Doctor may order a blood smear made from EDTA- anticoagulated blood many reasons why your doctor order... Proper clarity one time, a blog truly devoted to the Paramedical personnels risk... Leaving about 1 cm margins VACUTAINER METHOD smear • specimen:  peripheral blood.. Smear be prepared for studying the morphology of the smears EDTA- anticoagulated blood clean. Thick blood smears are used for the identification of microbial agents is then and.