Light-dependent N-terminal phosphorylation of LHCSR3 and LHCB4 are interlinked in Chlamydomonas reinhardtii. 72 • The electrons from cytochrome b 6 f are passed to another mobile electron carrier, a water-soluble, copper-containing, peripheral membrane protein called plastocyanin, situated on the luminal side of the thylakoid membrane • Plastocyanin carries electrons to the luminal side of PSI, where they are transferred to P700 +, the positively charged reaction-center pigment of PSI. 2020 Mar 24;11:318. doi: 10.3389/fpls.2020.00318. Taylor RM, Sallans L, Frankel LK, Bricker TM. mBio. 2018 Jul;137(1):141-151. doi: 10.1007/s11120-018-0485-0. USA.gov. Purification and crystallization of the cytochrome b6f complex in oxygenic photosynthesis. and to the corresponding domain in the 2.6 Å crystal structure of intact rat P450R. Consequences of the structure of the cytochrome b6f complex for its charge transfer pathways. 13. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Yan J, Cramer WA (2003) Functional insensitivity of the cytochrome b6f complex to structure changes in the hinge region of the Rieske iron-sulfur protein. AA presumably fails to ligate with haem b h, at variance with cytochrome bc 1 complex, owing to a specific Qi-site occupation in b 6 f . PCC 6803. NIH 2006 May-Jun;1757(5-6):339-45. doi: 10.1016/j.bbabio.2006.04.020. COVID-19 is an emerging, rapidly evolving situation. Please enable it to take advantage of the complete set of features! NLM 2011 Mar;141(3):289-98. doi: 10.1111/j.1399-3054.2010.01434.x. The reaction rate of PQ reduction of the investigated complexes in liposomes was not significantly influenced by the addition of free FNR or ferredoxin. Hui Bin Zhang, J. Whitelegge, W. Cramer; The Journal of biological chemistry; 2001; Corpus ID: 15547698. J Biol Chem 278:20925–20933 CrossRef PubMed Google Scholar. eCollection 2014. Moreover, we have shown that PQ can be non-enzymatically reduced by ascorbate in liposomes and this reaction might be involved in non-photochemical reduction pathways of the PQ-pool in chloroplasts.  |  J Biol Chem 276(41):38159–38165. 2019 Sep;99(5):877-894. doi: 10.1111/tpj.14368. Purified detergent-soluble cytochromeb6f complex from chloroplast thylakoid membranes (spinach) and cyanobacteria (Mastigocladus laminosus) was highly… 2001. 2019 Nov;575(7783):535-539. doi: 10.1038/s41586-019-1746-6. Ferredoxin:NADP+ oxidoreductase is a subunit of the chloroplast cytochrome b6f complex. Epub 2013 Jul 19. The b6 fcomplex occupies an electrochemically central position in the noncyclic or … LFNR1 and LFNR2 were found to associate with the cytochrome b6f complex following its partial purification. Natively oxidized amino acid residues in the spinach cytochrome b. 1), in which ferredoxin (12–16) and ferredoxin: NADP+ reductase (FNR) (17–20) can function as electron transfer intermediates between PSI and the b 6 f complex, as does a small very basic peptide, ‘‘PGR5’’ (21), presumably for reasons of structure. The decline in ferredoxin abundance under high temperature could be in response to the cell prioritizing Fe for other purposes, e.g. Highly Cited. It was inferred that the b6f complex with bound FNR, one equivalent per monomer, provides the membrane protein connection to the main electron transfer chain for ferredoxin-dependent cyclic electron transport. Dibrova DV, Cherepanov DA, Galperin MY, Skulachev VP, Mulkidjanian AY. The cytochrome b6f complex links photosystems II and I. Ferredoxin . 8. The absence of FNR in the cyanobacterial complex did not impair decyl-plastoquinol-ferricyanide activity. Methods Mol Biol. PSII. Non-photochemical reduction of thylakoid photosynthetic redox carriers in vitro: relevance to cyclic electron flow around photosystem I? J Biol Chem. The order of action is water – plastoquinone oxidoreductase (Photosystem II), plastoquinone – plastocyanin oxidoreductase (cytochrome b6f ), and plastocyanin – ferredoxin oxidoreductase (Photosystem I). Transfers the electrons to the Ferredoxin NADP Reductase (FNR) protein. USA.gov. The reaction is analogous to the reaction Is this relevant? National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The cytochrome b6f complex is responsible for "non-cyclic" (1) and "cyclic" (2) electron transfer between two mobile redox carriers, plastoquinone (QH2) and plastocyanin (Pc):  |  Cryo-EM structure of the spinach cytochrome b, Structural and functional contributions of lipids to the stability and activity of the photosynthetic cytochrome. Ferredoxin:NADP+ oxidoreductase is a subunit of the chloroplast cytochrome b6f complex. Purification and crystallization of the cyanobacterial cytochrome b6f complex. doi: 10.1128/mBio.00669-19. Structure–Function of the Cytochrome b ... (Fig. Nature. Phytochemistry. Adds two electrons and a hydrogen atom to NADP which creates NADPH . The label appears as 18 O 2 H 2 18 O + CO 2 → (light) (CH 2 O) 18 O 2 12. 2004;274:67-78. doi: 10.1385/1-59259-799-8:067. 1997. doi: 10.1074/jbc.M105454200 CAS Front Plant Sci.  |  Clipboard, Search History, and several other advanced features are temporarily unavailable. An additional high molecular weight (M(r) approximately 35,000) polypeptide in the chloroplast complex was seen in SDS-polyacrylamide gel electrophoresis at a stoichiometry of approximately 0.9 (cytochrome f)(-1). HHS Biochim Biophys Acta. Tweaking Photosynthesis: FNR-TROL Interaction as Potential Target for Crop Fortification. Purified detergent-soluble cytochrome b6f complex from chloroplast thylakoid membranes (spinach) and cyanobacteria (Mastigocladus laminosus) was highly active, transferring 300-350 electrons per cyt f/s. Biochim Biophys Acta. Ferredoxin-thioredoxin reductase EC 1.8.7.2, systematic name ferredoxin:thioredoxin disulfide oxidoreductase, is a [4Fe-4S] protein that plays an important role in the ferredoxin/thioredoxin regulatory chain. Learn all about P680 chlorophyll a. copper containing protein, functions as an electron transfer agent between cytochrome b6f and PSI. 2006 May-Jun;1757(5-6):339-45. doi: 10.1016/j.bbabio.2006.04.020. Zhang H, Whitelegge JP, Cramer WA (2001) Ferredoxin:NADP+ oxidoreductase is a subunit of the chloroplast cytochrome b6f complex. Would you like email updates of new search results? doi: 10.1371/journal.pone.0105952. This site needs JavaScript to work properly. Photochem Photobiol. Photosynth Res. and shown to be very similar both to the global fold in soln. Zhang H, Whitelegge JP, Cramer WA (2001) Ferredoxin: NADP + oxidoreductase is a subunit of the chloroplast cytochrome b 6 f complex. Bhaduri S, Zhang H, Erramilli S, Cramer WA. The cytochrome b6f complex occupies a central position in the sequence of photosynthetic electron transport carriers, oxidizing plastoquinol (PQH 2) and providing the electron transfer connection between the two reaction center complexes, PS II and PS I, to which H + transfer is coupled, thus contributing to the trans-membrane. Biochim Biophys Acta. PLoS One.  |  The extra polypeptide did not stain for heme and was much more accessible to protease than cytochrome f. Electrospray ionization mass spectrometry of CNBr fragments of the 35-kDa polypeptide was diagnostic for ferredoxin:NADP+ oxidoreductase (FNR), as were antibody reactivity to FNR and diaphorase activity. Plastoquinones are effectively reduced by ferredoxin:NADP+ oxidoreductase in the presence of sodium cholate micelles. Purified detergent-soluble cytochrome b6f complex from chloroplast thylakoid membranes (spinach) and cyanobacteria (Mastigocladus laminosus) … 258, 10348‐10354] is shown to be ferredoxin‐NADP + oxidoreductase (EC 1.18.1.2) on the basis of immunoreactivity, amino acid analysis, and pattern of cleavage by cyanogen bromide. 2011;684:65-77. doi: 10.1007/978-1-60761-925-3_7. Ferredoxin:NADP+ oxidoreductase bound to cytochrome b₆f complex is active in plastoquinone reduction: implications for cyclic electron transport. The photosynthetic electron transfer system consists of the electron carriers such as ferredoxin, plastocyanin, cytochromes, plastoquinone, and manganese. Epub 2006 May 4. 2014 Dec;1837(12):1944-1954. doi: 10.1016/j.bbabio.2014.09.005. Structure-function of the cytochrome b6f complex. The 37‐kDa non‐heme component in spinach cytochrome b‐f complex prepared from EDTA‐washed thylakoids [(1983) J. Biol. Highly Cited. 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